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A soft technology to improve survival and reproductive performance of Litopenaeus stylirostris by counterbalancing physiological disturbances associated with handling stress ArchiMer
Wabete, Nelly; Chim, Liet; Pham, Dominique; Lemaire, Pierrette; Massabuau, J.
The consequences of handling stress (fishing, transfer, eyestalk ablation) on shrimp broodstock are poorly documented. The weakness of farmed shrimp, Litopenaeus stylirostris, during winter is a major problem in New Caledonia, because of seasonal climate (tropical-sub-temperate). The transfer of broodstock in winter from earthen outdoor ponds to indoor maturation tanks in the hatchery (T=20 degrees C, Salinity=35 parts per thousand, fed shrimp) usually leads, after 48 h, to high mortality (up to 70%). Eyestalk ablation to induce ovarian maturation in females leads to further mortality. Starting from a background analysis of physiological disturbances (initial osmoregulatory imbalance) associated with handling stress (Wabete, N., Chim, L., Lernaire, P.,...
Tipo: Text Palavras-chave: Eyestalk ablation; Spawning; Handling stress; Broodstock; Litopenaeus stylirostris; Shrimp.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1880.pdf
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Combined effect of temperature and salinity on osmoregulation of juvenile and subadult Penaeus stylirostris ArchiMer
Lemaire, Pierrette; Bernard, Eric; Martinez-paz, J; Chim, Liet.
In New Caledonia, the "syndrome 93," which results in mass mortalities of farmed shrimp Penaeus stylirostris, occurs during the transitional and the cold seasons. The transitional season is characterized by an important fall in the average water temperature from 28 to 19 degreesC in 2 weeks. Besides this marked temperature fall, there are also nyctemeral temperature changes of up to 7 degreesC in 12 h. The cold season is characterized by an average water temperature of 19 degreesC with extreme lows of 14 degreesC. The objective of this study was to determine the effect of temperature and its variations on the osmoregulatory capacity of juvenile (7-10 g average weight) and subadult (25 g average weight) shrimps subjected to conditions of different salinity....
Tipo: Text Palavras-chave: Temperature; Salinity; Osmoregulatory capacity; Penaeus stylirostris; Shrimp.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-395.pdf
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Contribution à l'amélioration des survies et performances de reproduction de L. stylirostris en saison fraîche en Nouvelle-Calédonie ArchiMer
Pham, Dominique; Wabete, Nelly; Lemaire, Pierrette; Mailliez, Jean-rene; Broutoi, Francis; Chim, Liet.
Since 1993, the New Caledonian shrimp industry has been faced with cold season mortality outbreaks of multifactorial origin. This pathology called “Syndrome 93” affects as well animals in grow-out ponds as broodstock after their transfer in maturation room, with up to 35% of mortality in 48 hours. From literature data and physiological studies on Litopenaeus stylirostris, several combinations of three abiotic parameters (temperature, salinity and feeding) have been tested during the breeders stocking period. This research allowed us to lower stress factors impact and to propose a transfer protocol which leads to an optimal physiological comfort of the animal. Results showed that survival rate is improved and spawning rates are twice higher. These findings...
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Ano: 2008 URL: http://archimer.ifremer.fr/doc/00118/22970/20800.pdf
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Could a diet enriched with n-3 highly unsaturated fatty acids be considered a promising way to enhance the immune defences and the resistance of Penaeid prawns to environmental stress? ArchiMer
Chim, Liet; Lemaire, Pierrette; Delaporte, Maryse; Le Moullac, Gilles; Galois, Robert; Martin, Jean-louis.
The prawn Penaeus stylirostris (Stimpson), when fed for 28 days with n-3 highly unsaturated fatty acid (HUFA)-enriched feed pellets, demonstrated an enhanced resistance to variations in environmental parameters (a decrease in temperature and salinity over a 4-day period from 28 degreesC to 17 degreesC and from 35 parts per thousand to 10 parts per thousand respectively) and an improvement in their immune defence potential, i.e. increased agglutination titre of plasma and increased respiratory burst of haemocytes.
Tipo: Text Palavras-chave: Immunostimulation; Osmoregulation; Environment; Stress; Essential fatty acids; Crustaceans.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-606.pdf
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DEDUCTION : A research project for shrimp farming sustainability in New-Caledonia ArchiMer
Beliaeff, Benoit; Chim, Liet; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Walling, Emilie; Ansquer, Dominique; Brun, Pierre; Castex, Mathieu; Coatanea, Denis; Courties, Claude; De Lorgeril, Julien; Dufour, Robert; Frappier, Julie; Goarant, Cyrille; Huber, Matthias; Lemaire, Pierrette; Lemonnier, Hugues; Loubersac, Lionel; Le Roux, Frederique; Lucas, Ronan; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Ramage, Yannick; Soulard, Benoit; Vic, Marlene; Vourey, Elodie; Wabete, Nelly.
New Caledonian shrimp farming feasibility studies started in the mid-seventies. Today ca. 2000 tons of Litopenaeus stylirostris, introduced in 1978, are semi-intensively produced each year. Since fifteen years, production has been impacted by two seasonal vibriosis, the “Summer” syndrome caused by Vibrio nigripulchritudo in relation to pond bottom and water column quality, and the “Winter Syndrome” caused by Vibrio penaeicida in relation to drastic temperature drops . With the ambition of elucidating processes possibly leading to mortality outbreaks in ponds, Ifremer has been conducting a research program since 2003 under the DESANS project (2003-2006) and the present DEDUCTION project, equally funded by the South and North Provinces and by the Government...
Tipo: Text Palavras-chave: Shrimp farming; Technical and scientific support; Vibriosis; Water quality; Pathology; Ecophysiology; Genetics; Zoosanitary monitoring and procedures; Multifactorial and multidisciplinary approach.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00143/25438/23591.pdf
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Effect of dietary probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress status of shrimp Litopenaeus stylirostris ArchiMer
Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet.
in this study we evaluated the effects of the probiotic Pediococcus acidilactici MA18/5M on shrimp, Litopenaeus stylirostris (also called Penaeus stylirostris), first on antioxidant defences and secondly on the oxidative stress status in the shrimps' haemolymph and digestive gland. We conducted two experiments with the same protocol in which shrimps were fed two diets for three weeks: a control diet and a probiotic diet containing 1 g of live P. acidilactici MA18/5M kg(-1). In the first experiment, the shrimps were found to be healthy over the trial period; no mortalities and non-detectable signs of infection were recorded. These resulted in high final survival rates (above 90% in both treatments). On the other hand, during the second trial, carried out at...
Tipo: Text Palavras-chave: Vibriosis; Antioxidant defences; Oxidative stress; Probiotic; Shrimps.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6780.pdf
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Effect of probiotic Pediococcus acidilactici on antioxidant defences and oxidative stress of Litopenaeus stylirostris under Vibrio nigripulchritudo challenge ArchiMer
Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly; Chim, Liet.
Antioxidant defences and induced oxidative stress tissue damage of the blue shrimp Litopenaeus stylirostris, under challenge with Vibrio nigriputchritudo, were investigated for a 72-h period. For this purpose, L stylirostris were first infected by immersion with pathogenic V. nigripulchritudo strain SFn1 and then antioxidant defences: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), Total antioxidant status (TAS), glutathiones and induced tissue damage (MDA and carbonyl proteins) were determined in the digestive gland at 0, 12, 24, 48 and 72 h post-infection (h.p.i.). In the meantime, TAS was also measured in the blood. Infection level of the shrimps during the challenge was followed by determining V. nigripulchritudo prevalence...
Tipo: Text Palavras-chave: Probiotic; Pediococcus acidilactici; Shrimp; Oxidative stress; Antioxidant status; Vibrio; Immersion challenge.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00003/11419/7984.pdf
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Etude de la charge énergétique adénylique sur deux espèces aquacoles les crustacés Penaeus stylirostris et Penaeus vannamei et l'huître perlière Pinctada margaritifera ArchiMer
Lemaire, Pierrette.
La charge énergétique adénylique (CEA) est le reflet de l'énergie disponible dans les cellules. Ses variations ont été recherchées comme indice physiologique pour détecter un stress chez les crevettes péneides P.  vannamei et P. stylirosrtis et chez l' huître perlière Pinctada margaririfera. L'étude a porté dans un premier temps, sur la recherche des conditions optimales de préparation des échantillons aussi bien pour les crevettes que pour la nacre et dans un second temps sur des variations dues aux stress. La plupart des stress induits (hypoxie, vibriose) ne provoquent pas de variations significatives de la charge énergétique des crevettes p>0.05. Chez l'huître perlière une émersion à 20°C, 30°C et 40°C(p <0.05) ou une baisse de salinité ( 10%o)...
Tipo: Text Palavras-chave: Aquaculture tropicale; Péneides; Mollusques; Charge énergétique adénylique; Stress.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00436/54785/56239.pdf
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Evaluation of floating cages as an experimental tool for marine shrimp culture studies under practical earthen pond condition ArchiMer
Castex, Mathieu; Chim, Liet; Lemaire, Pierrette; Wabete, Nelly; Pham, Dominique.
Scientific and technical studies carried out under semi-intensive farming conditions, with large earthen ponds (Fig 1), to assess the effects of treatments on zootechnical results are highly complex due to the many logistical, economic and/or experimental constraints involved. In our study, we evaluated the use of floatingcages (Paquotte et al., 1998) immersed in earthen ponds as an experimental tool in order to design future powerful and economical expe-riments under semi-intensive farming conditions.
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00197/30872/29240.pdf
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Evaluation of floating cages as an experimental tool for marine shrimp culture studies under practical earthen pond conditions ArchiMer
Chim, Liet; Castex, Mathieu; Pham, Dominique; Brun, Pierre; Lemaire, Pierrette; Wabete, Nelly; Schmidely, P; Mariojouls, C.
The New Caledonia blue shrimp Litopenaeus stylirostris is commercially produced under semi-intensive rearing conditions. The size of the farming earthen ponds (5–10 ha) and the subsequent production constraints make it difficult to use them as experimental units for random experiments. Moreover, since every pond has its own characteristics, ponds' effects cannot be ruled out, thus making it hard to define true replicates. In order to design future experiments under conditions resembling those used in production, we evaluated the possible use of floating cages as experimental units with the aim of assessing treatment effects with a reasonable statistical power. To this end, two sets of floating cages were placed respectively in two different ponds in a...
Tipo: Text Palavras-chave: Statistical power; Replicates; Pond experimentation; Floating cages; Litopenaeus stylirostris.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4363.pdf
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Feed intake, molt frequency, tissue growth, feed efficiency and energy budget during a molt cycle of mud crab juveniles, Scylla serrata (Forskål, 1775), fed on different practical diets with graded levels of soy protein concentrate as main source of protein ArchiMer
Nguyen, Ngoc Thi Bich; Chim, Liet; Lemaire, Pierrette; Wantiez, Laurent.
There has been growing interest in the development of mud crab aquaculture in New Caledonia. However, for this to become established at a commercial level, a cost-effective formulated feed based on internationally-available ingredients needs to be developed. We have evaluated the optimal dietary protein content for juvenile crabs, Scylla serrata (Forskål, 1775), using a series of diets with a protein content ranging from 27 to 49% and soy protein concentrate (SPC) as the main protein source. For this purpose, 54 individually housed crabs were allocated to five dietary treatments (n = 10 or 11). The crabs were fed ad libitum, for 81 days with the allocated diets. The apparent digestibilities of dry mater, crude protein and energy were high (96.2-97.3%),...
Tipo: Text Palavras-chave: Scylla serrata; Tissue growth; Feed efficiency; Protein requirement; Soy protein concentrate; Energy budget.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00211/32196/30630.pdf
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Floating cages as an experimental tool for shrimp culture studies: first attempts to check their reliability ArchiMer
Pham, Dominique; Castex, Mathieu; Wabete, Nelly; Lemaire, Pierrette; Brun, Pierre.
The first aim of this study was to evaluate on a technical basis the rearing of shrimps in floating cages set up in earthen ponds. Shrimps reared in and outside the cages presented comparable growths and survival rates. Then it was concluded that shrimp culture in floating cages did not present zootechnical biases comparing to pond culture. The second objective of this study was to assess the present method as a statistical point of view. We found that for expected differences of 20% to the control mean, 3 and 6 floating cages per treatment will be reasonable to determined statistical differences for growth parameters and survival. Furthermore, we showed a significant pond effect as regard as survival and growth between both sets of cages. This results...
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Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-4100.pdf
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Improved Nutrition Enhances Immune Competence, Disease Resistance In Penaeid Shrimp ArchiMer
Coutteau, Peter; Chim, Liet; Saulnier, Denis; Lemaire, Pierrette.
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Ano: 2001 URL: http://archimer.ifremer.fr/doc/00174/28481/26821.pdf
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Oxidative stress studies applied to the farmed shrimp Litopenaus stylirostris in new caledonia ArchiMer
Chim, Liet; Castex, Mathieu; Lemaire, Pierrette; Wabete, Nelly.
The study of the antioxidant status and oxidative stress in shrimp L. stylirostris make it possible to assess risk factors (temperature, shrimp size, pathogen, ..) associated with shrimp farming. From a practical view, this research should lead to zootechnical and/or nutritional recommendations to improve the antioxidant status of the shrimp during their growout stage in farm.
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00197/30875/29243.pdf
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Probiotic P-acidilactici application in shrimp Litopenaeus stylirostris culture subject to vibriosis in New Caledonia ArchiMer
Castex, Mathieu; Chim, Liet; Pham, Dominique; Lemaire, Pierrette; Wabete, Nelly; Nicolas, Jean-louis; Schmidely, P; Mariojouls, C.
We studied the effects of a lactic acid bacterium, Pediococcus acidilactici (strain MA 18/5M, CNCM), as a dietary probiotic on growth performance and some nutritional and microbiological aspects of the shrimp Litopenaeus stylirostris. Experimental shrimp culture was carried out over 10 weeks, using floating cages of 14 m(2) each set in earthen ponds, in a New Caledonia farm affected by "Summer syndrome", a septicaemic vibriosis caused by Vibrio nigripulchritudo. The experiment design testing probiotic-coated pellets against control was replicated in two earthen ponds. High mortalities, characteristic of Summer syndrome, were observed during the first 2 weeks of the trial. The probiotic improved production in the treated cages from both ponds with increases...
Tipo: Text Palavras-chave: Vibriosis; Floating cages; Litopenaeus stylirostris; Pediococcus acidilactici; Probiotic.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4079.pdf
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Projet Déduction 2007-2010. Bilan par action et annexes ArchiMer
Beliaeff, Benoit; Laugier, Thierry; Chim, Liet; Coatanea, Denis; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Lemaire, Pierrette; Lemonnier, Hugues; Morvan, Patrice; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Soulard, Benoit; Wabete, Nelly; Walling, Emilie; Ansquer, Dominique; Castex, Mathieu; Lucas, Ronan; Reynaud, Yann; Ifremer, LEAD-NC,.
Ce document constitue le bilan détaillé des résultats du projet DEDUCTION (2007-2010) , action par action. Le projet DEDUCTION "DEveloppement DUrable de la Crevetticulture, Traitement de l’Information et Observatoire du système en Nouvelle-Calédonie" est un projet de recherche et de développement en soutien à la filière crevetticole néo-calédonienne. C'est un programme très horizontal et pluridisciplinaire en poursuivant et développant les ouvertures scientifiques, techniques et thématiques établies à la faveur de DESANS. Son ossature s’articule autour de 4 actions : 3 actions de recherche et une action transversale devant faciliter les interfaces entre la recherche et sa finalité, entre les chercheurs et les partenaires locaux (GFA, Provinces,...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00199/31006/35663.pdf
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Three years of experimental and applied research on the use of dietary probiotic Pediococcus acidilactici in shrimp culture : overview of the main results ArchiMer
Castex, Mathieu; Chim, Liet; Lemaire, Pierrette; Wabete, Nelly.
In recent years the biological control of diseases by environmental friendly methods such as probiotics has become an important subject of investigation in aquaculture research. Probiotics are live microorganisms which when administrated in adequate amounts confer a health benefit on the host . In the last 4 years, IFREMER in collaboration with Lallemand company, have carried out researchs in New Caledonia with the aim to assess the probiotic strain P. acidilactici MA 18/5M on farmed shrimp Litopenaeus stylirostris.This project started in 2005 demonstrated the beneficial effects of the probiotic Pediococcus acidilactici dietary supplementation on the nutrition and the health status of peneid shrimps and highlighted that its use as probiotic can be part of...
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00197/30873/29241.pdf
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